In Vitro Antifungal Activity of Eco-Enzyme Derived from Horticultural Organic Waste Against Candida albicans

Authors

  • Najla Lubis Universitas Pembangunan Panca Budi, Medan, Indonesia Author
  • Ruth Riah Ate Tarigan Universitas Pembangunan Panca Budi, Medan, Indonesia Author
  • Dwi Hayati Universitas Pembangunan Panca Budi, Medan, Indonesia Author

DOI:

https://doi.org/10.64803/cessmuds.v1.3
   

Keywords:

eco-enzyme, Candida_albicans, antifungal activity, organic_waste, horticulture

Abstract

This study aimed to evaluate the antifungal activity of eco-enzyme (EE) derived from various horticultural organic wastes against Candida albicans. Two formulations were prepared: Bioz1 (pineapple peel, orange, papaya, starfruit, kuini, and mango) and Bioz2 (pineapple, orange, banana corm, noni, and kuini). Both were fermented for 100 days. The antifungal activity was tested in vitro using the disc diffusion method at concentrations of 1:0, 1:10, 1:50, and 1:100 (EE: distilled water). Results indicated that Bioz1 at 1:50 exhibited the highest inhibition zone (7.38 mm, medium category), while Bioz2 at 1:0 showed the strongest activity (8.16 mm, medium category). Dilution at 1:10 in Bioz2 was the most effective concentration for inhibiting fungal growth. These findings highlight the potential use of eco-enzyme as an environmentally friendly natural disinfectant against pathogenic fungi

References

Armaniar, A., Sulardi, S., & Wibowo, F. (2023). Response of Liquid Organic Fertilizer Application from Sword Fern Weeds and Goat Manure on Growth and Production of Green Beans ( Vigna radiata L ). 9(November), 717–726.

Benny, N., Shams, R., Dash, K. K., Pandey, V. K., & Bashir, O. (2023). Recent trends in utilization of citrus fruits in production of eco-enzyme. Journal of Agriculture and Food Research, 13(January), 100657. https://doi.org/10.1016/j.jafr.2023.100657

Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error. Applied Microbiology, 22(4), 659–665. https://doi.org/10.1128/aem.22.4.659-665.1971

Lubis, N., Damayanti, R., & Wardani, S. (2024). Antibacterial Activity of Ecoenzymes with Various Dilutions Against Escherichia Coli. 1St International Conference of Digital Sciences and Engineering Technology, 122–127.

Lubis, N., Wasito, M., Marlina, L., Girsang, R., & Wahyudi, H. (2022). Respon Pemberian Ekoenzim dan Pupuk Organik Cair Terhadap Pertumbuhan dan Produksi Bawang Merah (Allium ascalonicum L.). Agrium, 25(2), 107–115. https://doi.org/10.30596/agrium.v25i2.10354 107

Luta, A. D., Siregar, M., Syam, F. H., Feruzi, Y., & Syafridawani, J. (2022). Efektivitas Pemberian Media Tanam dan Ekoenzim Pada Pertumbuhan Bawang Merah ( Allium ascalonicum L .). Seminar Nasional UNIBA Surakarta, 275–277.

Mavani, H. A. K., Tew, I. M., Wong, L., Yew, H. Z., Mahyuddin, A., Ghazali, R. A., & Pow, E. H. N. (2020). Antimicrobial efficacy of fruit peels eco-enzyme against Enterococcus faecalis: An in vitro study. International Journal of Environmental Research and Public Health, 17(14), 1–12. https://doi.org/10.3390/ijerph17145107

Ramadani, A. H., Karima, R., & Ningrum, R. S. (2022). Antibacterial Activity of Pineapple Peel (Ananas comosus) Eco-enzyme Against Acne Bacterias (Staphylococcus aureus and Prapionibacterium acnes). Indo. J. Chem. Res., 9(3), 201–207. https://doi.org/10.30598//ijcr

Rusdianasari, Syakdani, A., Zaman, M., Sari, F. F., Nasyta, N. P., & Amalia, R. (2021). Production of Disinfectant by Utilizing Eco-enzyme from Fruit Peels Waste. International Journal of Research in Vocational Studies (IJRVOCAS), 1(3), 01–07. https://doi.org/10.53893/ijrvocas.v1i3.53

Sitepu, sri mahareni, & Refnizuida. (2023). Peningkatan Produksi Bawang Merah (Allium asclonicum L.) Akibat Pemberian NPK Fermentasi Berbagai Jenis Limbah Tanaman. Jurnal Agroplasma, 10(1), 345–350.

Wasito, M. (2023). Training on Making Eco Enzymes from Fruit Waste in Suka Damai Village , Kuala Sub-District , Langkat District. 2(2), 232–241.

Yong, Y. S., et al. (2019). Application of eco-enzyme in sustainable agriculture: A review. Journal of Cleaner Production, 234, 157–169

Downloads

Published

2025-09-29

Issue

Section

Articles

How to Cite

In Vitro Antifungal Activity of Eco-Enzyme Derived from Horticultural Organic Waste Against Candida albicans. (2025). Proceedings of The International Conference on Computer Science, Engineering, Social Science, and Multi-Disciplinary Studies, 1, 8-10. https://doi.org/10.64803/cessmuds.v1.3